Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Rockland Immunochemicals 10X PBST (0.2 M Potassium Phosphate 1.5 M Sodium Chloride, 0.5% (v/v) Tween-20, pH 7.2) - MB-075-1000
Phosphate Buffer Saline-Tween is commonly use in washing procedure while performing western-blot, ELISA and, more generally immuno-assays.
Sigma Aldrich Fine Chemicals Biosciences Tris Buffered Saline BioUltra, tablet (for 500 mL), pH 7.6 | MFCD00132476 | 10TAB
Tris Buffered Saline BioUltra, tablet (for 500 mL), pH 7.6 | MFCD00132476 | 10TAB
Kd Medical Inc PBS 10X, PH 7.4
Standard buffer for many biomolecular procedures. One 1 Liter bottle.
Hampton Research HR2-729/1.0 M HEPES pH 7.5 - 100 ml
HR2-729/1.0 M HEPES pH 7.5 - 100 ml. Please note items from this supplier are non-returnable.
Apexbio Technology LLC AO/PI Double Staining Kit 100T
The AO/PI Double Staining Kit is a reagent kit enabling rapid detection of cell viability This kit provides two nuclear dyes Acridine Orange (AO) and Propidium Iodide (PI) AO can penetrate intact cell membranes and stain viable cells green During apoptosis chromosomes become condensed or fragmented into differently sized segments In this scenario AO stains apoptotic cells densely with yellow-green fluorescence or as yellow-green fragments The other nuclear dye PI cannot pass through intact cell membranes therefore it does not stain viable or apoptotic cells in red but stains cells with compromised membranes (e g necrotic cells) When using these two dyes in combination viable cells exhibit green fluorescence apoptotic cells show dense varied-size yellow-green or orange fluorescence and necrotic cells exhibit strong red fluorescence
Research Products International Corp Phosphate Buffered Saline, 10X Solution, 500 Milliliters
PBS 10X Solution is a concentrated phosphate-buffered saline widely used in cell biology, immunohistochemistry, and molecular biology. It contains phosphate ions that maintain physiological pH and ionic strength, creating a stable environment for cells, tissues, and biomolecules. The 10X concentration allows for easy dilution to 1X for various applications such as washing, resuspending, or preparing samples. The phosphate buffer system resists pH changes, ensuring experimental consistency. PBS 10X is a reliable, versatile reagent that supports reproducible results in biological and biochemical workflows.
Research Products International Corp TRIS-Tricine-SDS Running Buffer 10X Solution, 1 Liter
10X Formulation:
Tricine: 1 M
SDS (pH 8.3): 1%
FUJIFILM BIOSCIENCES INC PBS 1X-Dulbecco's Phosphate Buffered Saline Solution - Liquid
PBS 1X Dulbecco's Phosphate Buffered Saline Solution can be used to provide a buffer system to maintain cell culture media in the physiological pH range, or to irrigate, transport, or dilute fluid while maintaining cell tonicity and viability.
Sigma Aldrich Fine Chemicals Biosciences Tris Acetate-EDTA buffer B1L
TRIS Tris Acetate-EDTA buffer B1L
BioMed Diagnostics An isotonic solution typically used for detection of the severe acute respiratory syndrome SARS-CoV-2. | Available in: 50 pack
This Saline Solution (0.85%) has been validated for the detection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that causes COVID-19 disease in humans. | Product Principle: 1.) Intended to aid in the process of collection and transport of human clinical specimen. 2.) Ensures a non-replicating competent status of SARS-CoV-2. 3.) Is aseptic and does not contain bacteria or fungi growth-inhibition agents. 4.) Validated to be compatible with the identification of SARS-CoV-2 using qRT-PCR technology | Detects: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). | Available in: 50 pack
Boston Bioproducts Inc MES Buffer (1 M, pH 6.0) - 250ml
MES Buffer [2- (N-morpholino) ethanesulfonic acid] is a zwitterionic Good's buffer used in biological and biochemical research. It maintains a stable pH within the range of 5.5 to 7.0 and is highly soluble in water. MES is commonly used as a running buffer for bis-tris gels, chromatography, and in protein purification. It is valuable for enzymatic reactions, and acts as a non-coordinating buffer in metal ion chemistry. It has minimal UV absorbance and is stable in various applications.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Bufer500ML
DULBECCOS Dulbeccos Phosphate Bufer500ML
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine HEPES or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.