Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

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3,421 – 3,435 of 3,489 results

3,421

Boston Bioproducts Inc Sodium Phosphate Buffer (0.2 M, pH 7.0) - 1L

The Sodium Phosphate Buffer is a versatile reagent with a high buffering capacity, commonly used across various scientific disciplines across biochemistry and molecular biology. This buffer has a pH range of 5.8 to 8.0. It's applications include protein analysis, where it supports solubility, stability, and activity. It is also compatible with biologically relevant metal ions that are significant for cellular or physiological conditions.

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3,422

LGC GENOMICS LLC (Lucigen) TE Buffer, 70 mLStandard buffer for storing nucleic acids (10 mM Tris-HCl [pH 7.5], 1 mM EDTA). To be used with the MasterPure Kits and any other nucleic acid extraction/purification kits.

This reagent is designed for use with the MasterPure™ kit protocols only.

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3,423

Sigma Aldrich Fine Chemicals Biosciences HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culture | 7365-45-9 | MFCD00006158 | 500g

HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culture | Purity: | MW: 238.3 | 7365-45-9 | MFCD00006158 | 500g

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3,424

Boston Bioproducts Inc MOPS Buffer (1 M, pH 7.4) - 500ml

The MOPS Buffer [3- (N-morpholino)propanesulfonic acid buffer] is a versatile zwitterionic Good's buffer used in biological research. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH, and maintains a pH range from 6.5 to 7.9. It is often used as a buffering agent in biology and biochemistry, and is recommended for polyacrylamide gel electrophoresis, as a running buffer, in separating RNA in agarose gels, and for protein purification chromatography.

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3,425

Sigma Aldrich Fine Chemicals Biosciences TRIS BUFFER 5KG

NC3661566 TRIS BUFFER 5KG

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3,426

Sigma Aldrich Fine Chemicals Biosciences Trizma(R) Pre-set crystals BioPerformance Certified, pH 7.0, average Mw 154.8 | 100G

Trizma(R) Pre-set crystals BioPerformance Certified, pH 7.0, average Mw 154.8 | 100G

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3,427

Cliawaived Inc Transport Tube, 3mL Phosphate Based Saline PBS (400 swabs and PBS Tubes per box)

Transport Tube, 3mL Phosphate Based Saline PBS. 102mm length vial contains 3ml of PBS solution. Includes mini-flocked swab with synthetic fibers and plastic shaft and break point at 80mm. INTENDED USEThe KaiBiLiTM Extended ViralTrans PBS is phosphate buffer saline which can maintain pH and osmotic balance, as well as provide cells with water and essential inorganic ions.SUMMARYAppropriate specimen collection and transport is important to accurate laboratory diagnosis of infectious disease. Not only personnel operating skills, but also a proper specimen collection and transport system are essential attributes for reliable diagnosis result. The KaiBiLiTM Extended ViralTrans PBS is suitable for collection, transport of clinical specimens.

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3,428

CUBE BIOTECH DIBMA Glycerol; 1 g

DIBMA GLYCEROL HEPES 1G

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3,429

TE Buffer, Tris-EDTA, 10X Solution, pH 7.6, Molecular Biology, Fisher BioReagents™

Pricing & Availability
Specifications

Color	Colorless
Physical Form	Liquid
Chemical Name or Material	Tris-EDTA (TE), 10X Solution
Grade	Molecular Biology
DNase	DNase free
Filtered Through	Filtered through a 5-micron filter and autoclaved.
ChemAlert Storage Symbol	Gray
Name Note	pH 7.6
CAS	7732-18-5
Protease	Protease free
Health Hazard 3	Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0
Health Hazard 2	CAUTION!
pH	7.4 to 8.1
Synonym	TE
Recommended Storage	RT

3,430

Fisher Scientific MOPS 10X Solution, Fisher BioReagents™

CAS: 1132-61-2 Molecular Formula: C7H15NO4S Molecular Weight (g/mol): 209.26 MDL Number: MFCD00006183 InChI Key: DVLFYONBTKHTER-UHFFFAOYSA-N Synonym: 3-n-morpholinopropanesulfonic acid,4-morpholinepropane sulfonic acid,3-morpholinopropanesulfonic acid,3-morpholinopropane-1-sulfonic acid,morpholinopropane sulfonic acid,unii-273bp63nv3,3-n-morpholino propanesulfonic acid,morpholinopropanesulfonic acid,3-[N-Morpholino]propane sulfonic Acid,4-morpholinepropanesulfonic acid PubChem CID: 70807 ChEBI: CHEBI:44115 IUPAC Name: 4-(3-sulfonatopropyl)morpholin-4-ium SMILES: [O-]S(=O)(=O)CCC[NH+]1CCOCC1

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Specifications

PubChem CID	70807
CAS	1132-61-2
Molecular Weight (g/mol)	209.26
ChEBI	CHEBI:44115
MDL Number	MFCD00006183
SMILES	[O-]S(=O)(=O)CCC[NH+]1CCOCC1
Synonym	3-n-morpholinopropanesulfonic acid,4-morpholinepropane sulfonic acid,3-morpholinopropanesulfonic acid,3-morpholinopropane-1-sulfonic acid,morpholinopropane sulfonic acid,unii-273bp63nv3,3-n-morpholino propanesulfonic acid,morpholinopropanesulfonic acid,3-[N-Morpholino]propane sulfonic Acid,4-morpholinepropanesulfonic acid
IUPAC Name	4-(3-sulfonatopropyl)morpholin-4-ium
InChI Key	DVLFYONBTKHTER-UHFFFAOYSA-N
Molecular Formula	C7H15NO4S

3,431

TBE Buffer Mix (Dry Powder Mix of Tris/Boric Acid/EDTA/Electrophoresis), Fisher BioReagents

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3,432

STE Buffer, Sodium Chloride-Tris-EDTA, 10X Solution, pH 8.0, Fisher BioReagents

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3,433

TAE Buffer, Tris-Acetate-EDTA, 25X Solution, Electrophoresis, Fisher BioReagents

CAS: 60-00-4

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3,434

TE Buffer, Tris-EDTA, 1X Solution, pH 7.6, Molecular Biology, Fisher BioReagents

Pricing & Availability
Specifications

Color	Colorless
Physical Form	Liquid
Chemical Name or Material	Tris-EDTA
Grade	Molecular Biology
DNase	DNase free
Filtered Through	Filtered through a 5-micron filter.
ChemAlert Storage Symbol	Gray
Name Note	1X Solution, pH 7.6
CAS	7732-18-5
Protease	Protease free
Health Hazard 3	Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0
Health Hazard 2	CAUTION!
pH	7.4 to 8.1
Synonym	TE
Recommended Storage	RT

3,435

CAPS (Fine White Crystals), Fisher BioReagents

CAS: 1135-40-6 Molecular Formula: C9H19NO3S Molecular Weight (g/mol): 221.32 MDL Number: MFCD00003837 InChI Key: PJWWRFATQTVXHA-UHFFFAOYSA-N Synonym: 3-Cyclohexylamino-1-propanesulfonic Acid PubChem CID: 70815 IUPAC Name: 3-(cyclohexylamino)propane-1-sulfonic acid SMILES: OS(=O)(=O)CCCNC1CCCCC1

Pricing & Availability
Specifications

PubChem CID	70815
CAS	1135-40-6
Molecular Weight (g/mol)	221.32
MDL Number	MFCD00003837
SMILES	OS(=O)(=O)CCCNC1CCCCC1
Synonym	3-Cyclohexylamino-1-propanesulfonic Acid
IUPAC Name	3-(cyclohexylamino)propane-1-sulfonic acid
InChI Key	PJWWRFATQTVXHA-UHFFFAOYSA-N
Molecular Formula	C9H19NO3S

Life Science Buffers

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